Answer

Which of the following is in the correct order for one cycle of polymerase chain reaction (PCR)

A) Denature DNA; add fresh enzyme; anneal primers; add dNTPs; extend primers.
B) Add fresh enzyme; denature DNA; anneal primers; add dNTPs; extend primers.
C) Anneal primers; denature DNA; extend primers.
D) Extend primers; anneal primers; denature DNA.
E) Denature DNA; anneal primers; extend primers.


Answer: E